Work Item
ASTM WK91650
New Test Method for the measurement of total bacteria in fuel and fuel associated water using quantitative polymerase chain reaction (qPCR)
1. Scope
This test method covers a protocol for capturing, preserving and purifying nucleic acids and quantifying total bacterial content in fuel and fuel-associated water using quantitative polymerase chain reaction.Keywords
qPCR; quantitative polymerase chain reaction; bacteria; fuel; microbiologyRationale
There is no standard for qPCR in fuel - several years ago a standard practice was published but lacks specific methodological details. qPCR is a more commonly used method for microbial detection and quantification and the industry would benefit from a standard method.
Work Item Status
Date Initiated: 07-17-2024
Technical Contact: Gregory Howard
Item: 000
Ballot:
Status: